Brain Endothelial Cells Express Cyclooxygenase-2 during Lipopolysaccharide-Induced Fever: Light and Electron Microscopic Immunocytochemical Studies

• DOI: 10.1523/jneurosci.18-16-06279.1998
• Publication Date: 2018-04-05T22:29:52Z
• Authors: Kiyoshi Matsumura; Chunyu Cao; Masashi Ozaki; Hiroshi Morii; Kazuhiko Nakadate; Yasuyoshi Watanabe
• Keywords: Lipopolysaccharides; Male; Time Factors; Fever; Staining and Labeling; Blotting, Western; Immunohistochemistry; Rats; 3. Good health; Isoenzymes; Microscopy, Electron; 03 medical and health sciences; 0302 clinical medicine; Cyclooxygenase 2; Prostaglandin-Endoperoxide Synthases; Cerebrovascular Circulation; Animals; Endothelium, Vascular; Rats, Wistar

Cyclooxygenase-2 (COX-2), a key enzyme in the biosynthesis of prostaglandins, is induced brain blood vessels by pyrogens, and its essential role fever has been hypothesized. In this study, we determined (1) type cells that express cyclooxygenase-2 lipopolysaccharide-treated rats, (2) precise relationship between time course protein expression these cells. Five hours after lipopolysaccharide injection (100 μg/kg, i.p.), cyclooxygenase-2-like immunoreactive were found parenchymal subarachnoidal vessels. vessels, immunoreactivity was restricted to perinuclear region endothelial as revealed laser confocal microscopy, double-immunofluorescence staining with an marker, immunoelectron microscopy. On other hand, distinct from microglia or perivascular/meningeal macrophages double immunostaining macrophage/microglia-specific antibodies. Cyclooxygenase-2-like first at 1.5 hr injection, which had not developed. After that, number followed similar course, both being highest 5 returning baseline 24 hr. These results demonstrate are primary sites where activation arachidonic acid cascade takes place during intraperitoneal lipopolysaccharide.