L-Type Ca2+Channels Are Essential for Glutamate-Mediated CREB Phosphorylation andc-fosGene Expression in Striatal Neurons

• DOI: 10.1523/jneurosci.19-15-06348.1999
• Publication Date: 2018-04-03T10:45:28Z
• Authors: Anjali Rajadhyaksha; Amy Barczak; Wendy Macı́as; Jean-Christophe L...; Susan E. Lewis; Christine Konradi
• Keywords: Kainate receptor; Long-term depression

The second messenger pathways linking receptor activation at the membrane to changes in nucleus are just beginning be unraveled neurons. work presented here attempts identify striatal neurons that mediate cAMP response element-binding protein (CREB) phosphorylation and gene expression NMDA activation. We investigated of transcription factor CREB, immediate early c-fos, induction a transfected reporter under transcriptional control CREB after stimulation ionotropic glutamate receptors. found neither AMPA/kainate receptors nor were able stimulate independently pathway led or c-fos expression. Instead, we saw consecutive from L-type Ca(2+) channels. involved relieving Mg(2+) block receptors, triggered opening activates is likely activated by entry through conclude primary glutamate-mediated signal transduction dependent on functional