Hippocampal GABAergic Synapses Possess the Molecular Machinery for Retrograde Nitric Oxide Signaling

• DOI: 10.1523/jneurosci.1912-07.2007
• Publication Date: 2007-07-25T17:39:30Z
• Authors: Eszter Szabadits; Csaba Cserép; Anikó Ludányi; István Katona; Javier Gracia-Llanes; Tamás F. Freund; Gábor Nyíri
• Keywords: Interneuron; Soluble guanylyl cyclase

Nitric oxide (NO) plays an important role in synaptic plasticity as a retrograde messenger at glutamatergic synapses. Here we describe that, hippocampal pyramidal cells, neuronal nitric synthase (nNOS) is also associated with the postsynaptic active zones of GABAergic symmetrical synapses terminating on their somata, dendrites, and axon initial segments both mice rats. The NO receptor oxide-sensitive guanylyl cyclase (NOsGC) present brain two functional subunit compositions: α 1 β 2 . expressed cells interneurons hippocampus. Using immunohistochemistry situ hybridization methods, that detectable only interneurons, which are always positive for well; however, labeled subunits. With double-immunofluorescent staining, found most cholecystokinin- parvalbumin-positive smaller proportion somatostatin- nNOS-positive positive. We parvalbumin- cholecystokinin-positive interneuron terminals establish or segments. Our results demonstrate NOsGC, composed subunits, selectively different types presynaptic terminals, it may serve produced postsynaptically by nNOS very same synapse.