Munc13-3 Superprimes Synaptic Vesicles at Granule Cell-to-Basket Cell Synapses in the Mouse Cerebellum

• DOI: 10.1523/jneurosci.2060-14.2014
• Publication Date: 2014-10-29T17:07:51Z
• Authors: Shimpei Ishiyama; Hartmut Schmidt; Benjamin H. Cooper; Nils Brose; Jens Eilers
• Keywords: Granule (geology)

Munc13-3 is a presynaptic protein implicated in vesicle priming that strongly expressed cerebellar granule cells (GCs). Mice deficient of ( −/− ) show an increased paired-pulse ratio (PPR), which led to the hypothesis increases release probability p r vesicles. In present study, we analyzed unitary synaptic connections between GCs and basket acute slices from wild-type mice. Unitary EPSCs recorded showed normal kinetics latency but significantly PPR fraction failures. A quantal analysis revealed neither charge single quanta nor binominal parameter N were affected by loss was almost halved . Neither Ca 2+ influx deletion replenishment readily releasable pool. However, high concentration EGTA reduction stronger We conclude responsible for additional step molecular and/or positional “superpriming” substantially efficacy -triggered release.