A Unique Role for Fyn in CNS Myelination

• DOI: 10.1523/jneurosci.21-06-02039.2001
• Publication Date: 2018-04-05T22:56:03Z
• Authors: Brian R. Sperber; Éilis A. Boyle-Walsh; Mark J. Engleka; Paul Gadue; Alan C. Peterson; Paul L. Stein; Steven S. Scherer; F. Arthur McMorris
• Keywords: FYN; LYN

We analyzed the role of Fyn tyrosine kinase in CNS myelination by using <i>fyn</i>^−/− null mutant mice, which express no protein. found a severe myelin deficit forebrain at all ages from 14 d to 1 year. The was maximal month age and similar regardless mouse strain background or whether it determined bulk isolation quantitation basic To determine cellular basis deficit, we counted oligodendrocytes tissue sections mice expressing oligodendrocyte-targeted β-galactosidase, used light electron microscopy examine number morphology myelinated fibers size structures. All these parameters were reduced in<i>fyn</i>^−/− mice. Unexpectedly, there regional differences deficit; contrast forebrain, cervical spinal cord exhibited reduction content, oligodendrocytes, fibers, nor delayed developmentally. that Src, but significant content mutants lacking Fyn-related kinases Yes, Lyn. Finally, investigated molecular features are required for single amino acid substitution, abolishes activity Fyn, resulted as great observed complete absence These results demonstrate plays unique myelination, one requires its activity.