The Ciliary Neurotrophic Factor/Leukemia Inhibitory Factor/gp130 Receptor Complex Operates in the Maintenance of Mammalian Forebrain Neural Stem Cells

• DOI: 10.1523/jneurosci.21-19-07642.2001
• Publication Date: 2018-04-06T19:15:28Z
• Authors: Takuya Shimazaki; Tetsuro Shingo; Samuel Weiss
• Keywords: Ciliary neurotrophic factor; Forebrain

The cytokines that signal through the common receptor subunit gp130, including ciliary neurotrophic factor (CNTF), interleukin-6, leukemia inhibitory (LIF) and oncostatin M, have pleiotropic functions in CNS development. Given restricted expression domain of CNTF α (CNTFR) developing forebrain germinal zone adult periventricular area, we examined putative role CNTFR/LIFR/gp130-mediated signaling regulating neural stem cell fate <i>in vivo</i> vitro</i>. Analysis of<i>LIFR</i>-deficient mice revealed a decreased level LIFR results reduction number cells. In <i>LIFR</i> heterozygote (+/−) mice, cells their progeny subependyma TH-immunoreactive neurons olfactory bulb were significantly reduced. Intraventricular infusion into mouse forebrain, absence or presence epidermal growth (EGF), enhanced self-renewal vivo</i>. Analyses EGF-responsive proliferating vitro</i> found inhibits lineage restriction to glial progenitors, which turn expansion number. These suggest supports maintenance cells, likely by suppressing progenitor fate.