An Activity-Dependent Neurotrophin-3 Autocrine Loop Regulates the Phenotype of Developing Hippocampal Pyramidal Neurons before Target Contact

• DOI: 10.1523/jneurosci.21-22-08789.2001
• Publication Date: 2018-04-05T00:11:56Z
• Authors: Hassan Boukhaddaoui; Victor Sieso; Frederique Scamps; Jean Valmier
• Keywords: Neurotrophin-3

Neurotrophin-3 (NT-3), its cognate receptor trkC, and voltage-gated calcium channels are coexpressed by embryonic pyramidal neurons before target contact, but their functions at this stage of development still unclear. We show here that, in vitro , anti-NT-3 anti-trkC antibodies blocked the increase, NT-3 reversed decrease number calbindin-D 28k -positive induced by, respectively, channel activations blockades. Similar results were obtained with single-neuron microcultures. In addition, inhibition downregulates extracellular levels high-density cultures. Moreover, electrophysiological experiments single-cell cultures reveal a tetrodotoxin-sensitive spontaneous electrical activity allowing activation. The mouse (−/−) mutation decreases 40% developing neurons, without affecting neuronal survival, both vivo . Thus, present strongly support that an activity-dependent autocrine loop provides local, intrinsic mechanism which, hippocampal pyramidal-like may regulate own differentiation, role be important during early CNS differentiation or after adult disruption.