Adult Rodent Neurogenic Regions: The Ventricular Subependyma Contains Neural Stem Cells, But the Dentate Gyrus Contains Restricted Progenitors

• DOI: 10.1523/jneurosci.22-05-01784.2002
• Publication Date: 2018-04-13T22:30:10Z
• Authors: Raewyn M. Seaberg; Derek van der Kooy
• Keywords: Subgranular zone; Neurosphere; Rostral migratory stream; Neuroepithelial cell

Neurogenesis persists in two adult brain regions: the ventricular subependyma and subgranular cell layer hippocampal dentate gyrus (DG). Previous work many laboratories has shown explicitly that multipotential, self-renewing stem cells are source of newly generated migrating neurons traverse rostral migratory stream incorporate into olfactory bulb as interneurons. These have been specifically isolated from subependyma, their properties self-renewal multipotentiality demonstrated vitro. In contrast, it is a widely held assumption "hippocampal" can be vitro hippocampus reside neurogenic represent new granule neurons, but this never tested directly. Primary isolates derived precise microdissection rodent regions were compared using very different commonly used culture methods: clonal colony-forming (neurosphere) assay monolayer system. Importantly, both these methods same conclusion: hippocampus-adjacent DG proper does not contain population resident neural cells. Indeed, although lateral ventricle other subependymal directly adjacent to exhibit long-term multipotentiality, separate neuronal glial progenitors with limited capacity present DG, suggesting neuron-specific multipotential throughout adulthood.