Estrogen Receptor β Mediates Rapid Estrogen Actions on Gonadotropin-Releasing Hormone NeuronsIn Vivo

• DOI: 10.1523/jneurosci.23-13-05771.2003
• Publication Date: 2018-04-13T22:30:28Z
• Authors: István M. Ábrahám; Seong-Kyu Han; Martin G. Todman; Kenneth S. Korach; Allan E. Herbison
• Keywords: Estrogen receptor beta

The gonadal steroid estrogen exerts an important modulatory influence on the activity of multiple neuronal networks. In addition to classical genomic mechanisms action, also poorly understood rapid, nongenomic effects neurons. To examine whether may exert rapid actions intracellular signaling within gonadotropin-releasing hormone (GnRH) neurons in vivo ,we examined phosphorylation status cAMP response element-binding protein (CREB) these cells after administration 17-β-estradiol ovariectomized (OVX) mice. percentage GnRH expressing phosphorylated CREB was increased more than sixfold ( p < 0.05) a time- and dose-dependent manner by estrogen, with increase first observed 15 min administration. A series vitro studies demonstrated that acted directly native phosphorylate CREB, but conjugated bovine serum albumin without effect. role receptors (ERs) evaluated using ER knock-out mice . effect normal ERα completely absent ERβ Finally, intact female revealed levels were equivalent those estrogen-treated OVX These observations demonstrate mediates direct phenotype, persist under physiological conditions. They provide evidence for brain