Possible Involvement of P2Y2Metabotropic Receptors in ATP-Induced Transient Receptor Potential Vanilloid Receptor 1-Mediated Thermal Hypersensitivity

• DOI: 10.1523/jneurosci.23-14-06058.2003
• Publication Date: 2018-04-06T19:50:50Z
• Authors: Tomoko Moriyama; Tohko Iida; Kimiko Kobayashi; Tomohiro Higashi; Tetsuo Fukuoka; Hideki Tsumura; Catherine Leon; Noboru Suzuki; Kazuhide Inoue; Christian Gachet; Koichi Noguchi; Makoto Tominaga
• Keywords: Dorsal root ganglion; P2Y receptor; Capsaicin

The capsaicin receptor transient potential V1 (TRPV1; also known as vanilloid 1) is a sensory neuron-specific ion channel that serves polymodal detector of pain-producing chemical and physical stimuli. It has been reported extracellular ATP potentiates the TRPV1 currents evoked by or protons reduces temperature threshold for its activation through metabotropic P2Y receptors in PKC-dependent pathway, suggesting could trigger sensation pain at normal body presence ATP. Here, we show ATP-induced thermal hyperalgesia was abolished mice lacking TRPV1, functional interaction between behavioral level. However, preserved P2Y1 receptor-deficient mice. Patch-clamp analyses using mouse dorsal root ganglion neurons indicated involvement P2Y2 rather than receptors. Coexpression mRNA with mRNA, but not determined rat lumbar DRG situ hybridization histochemistry. These data indicate importance nociception TRPV1.