Cycling of NMDA Receptors during Trafficking in Neurons before Synapse Formation

• DOI: 10.1523/jneurosci.2555-04.2004
• Publication Date: 2004-09-22T18:47:56Z
• Authors: Philip Washbourne; Xiao-Bo Liu; Edward G. Jones; A. Kimberley McAl...
• Keywords: Silent synapse

The trafficking of glutamate receptors in neurons is the utmost importance for synapse formation and synaptic plasticity. Recently, we demonstrated that both NMDA AMPA reside mobile transport packets are recruited rapidly independently to nascent synapses. Here, show a large proportion receptor clusters young cortical present on surface dendrites before synapses formed these surface-exposed mobile. Exocytosis dendritic occurs via SNARE [soluble n-ethylmaleimide-sensitive factor attachment protein (SNAP) receptor]-dependent SNAP-23-mediated mechanism. Endocytosis after exposure; >50% surface-labeled (NMDARs) endocytosed within 5 min. NMDARs transported along microtubules tubulovesicular organelles, as indicated by immunoelectron microscopy, associated with EEA1 (early endosomal antigen 1) SAP102 (synapse-associated 102), immunocytochemistry. Most surprisingly, cycle through plasma membrane formation. These results suggest novel model which during pauses movement while trafficking.