Ca2+Dynamics along Identified Synaptic Terminals inDrosophilaLarvae

• DOI: 10.1523/jneurosci.2665-06.2006
• Publication Date: 2006-11-22T18:11:37Z
• Authors: Gregory A. Lnenicka; Jeffrey Grizzaffi; Bomi Lee; Nidhi Rumpal
• Keywords: Dynamics

Changes in intracellular Ca 2+ concentration ([Ca ] i ) play an important role the function and plasticity of synapses. We characterized changes [Ca produced by action potentials (APs) along two identified motor terminals found on separate muscle fibers Drosophila larvae examined factors that influence amplitude duration residual signal. were able to measure transients both single APs AP trains using Oregon Green 488 BAPTA-1 streaming images at 20–50 Hz. The decay after or was well fit a exponential. For APs, transient rate similar boutons bottleneck regions much smaller axon. Also, single-AP inversely correlated with bouton width. During trains, increase became more uniform: difference axons reduced, not τ directly width for trains. one terminal, distal had larger than proximal ones, probably attributable greater influx boutons. Pharmacological studies showed clearance from these synaptic primarily extrusion plasma membrane ATPase (PMCA). Immunostaining larval high levels PMCA neuromuscular junction.