The Tetraspanin KAI1/CD82 Is Expressed by Late-Lineage Oligodendrocyte Precursors and May Function to Restrict Precursor Migration and Promote Oligodendrocyte Differentiation and Myelination

• DOI: 10.1523/jneurosci.3075-09.2009
• Publication Date: 2009-09-09T18:10:07Z
• Authors: Angeliki Mela; James E. Goldman
• Keywords: OLIG2; Remyelination; Tetraspanin

In the adult mammalian brain, oligodendrocyte progenitors can differentiate into mature oligodendrocytes during remyelination. Mechanisms that regulate migration and differentiation of are great importance in understanding normal development demyelinating/remyelinating conditions. a microarray analysis comparing neonatal O4-positive (+) cells, we found tetraspanin KAI1/CD82 is far more highly expressed O4 + cells than (Lin et al., 2009). CD82 metastasis suppressor, its expression often downregulated or lost advanced stages metastatic cancer. We hypothesized could be factor restricts promotes maturing oligodendrocytes. Western blot isolated confirms elevated levels CD82, which continues to as these become O1 vitro . rat white matter, coexpressed with CC1 olig2 but not NG2 GFAP. Immature forebrain subventricular zone (SVZ) infected vivo retrovirus constitutively expresses do remain immature either MBP myelinating matter zebrinII astrocytes cortex. Their from SVZ severely restricted. contrast, downregulation , using retroviral-expressed short hairpin RNAs (shRNAs), prevents their shRNA-expressing remained PDGF receptor α positive, became nonmyelinating GFAP astrocytes. thus appears critical molecule regulation progenitor myelination.