Segregation of Acute Leptin and Insulin Effects in Distinct Populations of Arcuate Proopiomelanocortin Neurons

• DOI: 10.1523/jneurosci.3118-09.2010
• Publication Date: 2010-02-17T18:19:56Z
• Authors: Kevin W. Williams; Lisandra O. Margatho; Charlotte E. Lee; Michelle Choi; Syann Lee; Michael M. Scott; Carol F. Elias; Joel K. Elmquist
• Keywords: Proopiomelanocortin; Leptin receptor

Acute leptin administration results in a depolarization and concomitant increase the firing rate of subpopulation arcuate proopiomelanocortin (POMC) cells. This rapid activation POMC cells has been implicated as cellular correlate effects on energy balance. In contrast to leptin, insulin inhibits activity some neurons. Several studies have described “cross talk” between within mediobasal hypothalamus via intracellular enzyme, phosphoinositol-3-kinase (PI3K). Interestingly, both regulate by PI3K; however, it is unclear whether are observed similar or distinct populations We therefore used dual label immunohistochemistry/ situ hybridization whole-cell patch-clamp electrophysiology map responsive Leptin-induced Fos neurons was localized separate from that express receptor. Moreover, acute responses were largely segregated subpopulations Collectively, these data suggest cross talk occurs network rather than individual