Netrin and Frazzled Regulate Presynaptic Gap Junctions at aDrosophilaGiant Synapse

• DOI: 10.1523/jneurosci.3183-13.2014
• Publication Date: 2014-04-16T16:38:01Z
• Authors: Brian O. Orr; Melissa A. Borgen; Phyllis M. Caruccio; Rodney K. Murphey
• Keywords: Netrin; Electrical Synapses

Netrin and its receptor, Frazzled, dictate the strength of synaptic connections in giant fiber system (GFS) Drosophila melanogaster by regulating gap junction localization presynaptic terminal. In mutant animals, coupling between a interneuron “jump” motor neuron was weakened dye these two neurons severely compromised or absent. cases which mutants displayed apparently normal anatomy, half specimens exhibited physiologically defective synapses (GF) reduced eliminated, suggesting that junctions were disrupted mutants. When we examined with antibodies to Shaking-B (ShakB) Innexin, they significantly decreased absent terminal GF. Frazzled loss function similar defects transmission, coupling, localization. These data are first show regulate placement presynaptically at synapse.