Distinct Subsets of Syt-IV/BDNF Vesicles Are Sorted to Axons versus Dendrites and Recruited to Synapses by Activity

• DOI: 10.1523/jneurosci.4515-11.2012
• Publication Date: 2012-04-18T16:39:06Z
• Authors: Camin Dean; Huisheng Liu; Thorsten Staudt; Markus A. Stahlberg; Siv Vingill; Johanna Bückers; Dirk Kamin; Johann Engelhardt; Meyer B. Jackson; Stefan W. Hell; Edwin R. Chapman
• Keywords: Active zone

BDNF plays a critical role in the regulation of synaptic strength and is essential for long-term potentiation, phenomenon that underlies learning memory. However, whether acts diffuse manner or targeted to specific neuronal subcompartments sites affect circuit function remains unknown. Here, using photoactivation syt-IV (a regulator exocytosis present on BDNF-containing vesicles) transfected rat hippocampal neurons, we discovered distinct subsets vesicles are axons versus dendrites not shared between these compartments. Moreover, syt-IV- BDNF-harboring recruited both presynaptic postsynaptic response increased activity. Finally, knockout mouse found necessary scaling changes network These findings demonstrate can be neurons suggest syt-IV-regulated secretion subject spatial control regulate site-specific manner.