Use-Dependent Activation of Neuronal Kv1.2 Channel Complexes

• DOI: 10.1523/jneurosci.4518-13.2015
• Publication Date: 2015-02-26T06:43:23Z
• Authors: Victoria A. Baronas; Brandon R. McGuin...; G. Stefano Brigidi; Rachel N. Gomm Ko...; Yury Y. Vilin; Robin Y. Kim; Francis C. Lynn; Shernaz X. Bamji; Runying Yang; Harley T. Kurata
• Keywords: Shaker; HEK 293 cells; SK channel

In excitable cells, ion channels are frequently challenged by repetitive stimuli, and their responses shape cellular behavior regulating the duration termination of bursts action potentials. We have investigated Shaker family voltage-gated potassium (Kv) subjected to with a particular focus on Kv1.2. Genetic deletion this subunit results in complete mortality within 2 weeks birth mice, highlighting critical physiological role for Kv1.2 exhibit unique property described previously as "prepulse potentiation," which activation depolarizing step facilitates subsequent pulse. study, we demonstrate that enables use-dependent during trains very brief depolarizations. Also, Kv subunits usually assemble into heteromeric central nervous system, generating diversity function sensitivity signaling mechanisms. other Kv1 channel types do not activation, but is conferred complexes containing even single subunit. This regulatory mechanism observed mammalian cell lines well primary cultures hippocampal neurons. Our findings illustrate persists may influence