Glioblastoma-Induced Attraction of Endogenous Neural Precursor Cells Is Associated with Improved Survival

• DOI: 10.1523/jneurosci.5118-04.2005
• Publication Date: 2005-03-10T01:59:20Z
• Authors: Rainer Glass; Michael Synowitz; Golo Kronenberg; Joo-Hee Walzlein; Darko S. Markovic; Li-Ping Wang; Daniela Gast; Jürgen Kiwit; Gerd Kempermann; Helmut Kettenmann
• Keywords: Nestin; Syngenic; Precursor cell

Neural precursor cells contribute to adult neurogenesis and limited attempts of brain repair after injury. Here we report that in a murine experimental glioblastoma model, endogenous neural precursors migrate from the subventricular zone toward tumor surround it. The association with syngenic grafts was observed, injecting red fluorescent protein-labeled G261 into caudate-putamen transgenic mice, which express green protein under promoter for nestin (nestin-GFP). Fourteen days inoculation, nestin-GFP surrounded tumors several cell layers expressed markers early noncommitted committed precursors. Nestin-GFP were further identified by characteristic membrane current pattern as recorded acute slices. 5-Bromo-2-deoxyuridine labeling dye tracing experiments revealed tumor-associated originated zone. Moreover, cultured explants zone, showed extensive tropism glioblastomas. Tumor-induced accumulation decreased age recipient; this correlated increased size shorter survival times aged mice. Coinjection improved time old mice level similar young Coculture suppressed rapid increase number, is glioblastoma, induced apoptosis. Our results indicate attract cells; presence antitumorigenic; cellular interaction decreases aging.