Endogenous Sequential Cortical Activity Evoked by Visual Stimuli

• DOI: 10.1523/jneurosci.5214-14.2015
• Publication Date: 2015-06-10T16:35:24Z
• Authors: L. Carrillo-Reid; J.-e. K. Miller; J. P. Hamm; J. Jackson; R. Yuste
• Keywords: Calcium imaging; Premovement neuronal activity; Photic Stimulation

Although the functional properties of individual neurons in primary visual cortex have been studied intensely, little is known about how neuronal groups could encode changing stimuli using temporal activity patterns. To explore this, we used <i>in vivo</i> two-photon calcium imaging to record populations awake mice presence and absence stimulation. Multidimensional analysis network allowed us identify ensembles defined as cells firing synchrony. These synchronous were themselves activated sequential patterns, which repeated at much higher proportions than chance triggered by specific such natural scenes. Interestingly, patterns also present recordings spontaneous without any sensory stimulation accompanied precise sequences single-cell level. Moreover, intrinsic dynamics be predict occurrence future ensembles. Our data demonstrate that recruit similar ones observed spontaneously, consistent with hypothesis already existing Hebbian cell assemblies predefined microcircuit substrate encodes percepts time.