Interplay among cGMP, cAMP, and Ca2+ in Living Olfactory Sensory Neurons In Vitro and In Vivo
Second messenger system
Soluble guanylyl cyclase
DOI:
10.1523/jneurosci.6722-10.2011
Publication Date:
2011-06-08T19:22:46Z
AUTHORS (6)
ABSTRACT
The mechanism of cGMP production in olfactory sensory neurons (OSNs) is poorly understood, although this messenger takes part several key processes such as adaptation, neuronal development, and long-term cellular responses to odorant stimulation. Many aspects the regulation OSNs are still unknown or highly controversial, its subcellular heterogeneity, coupling receptors downstream targets. Here, we have investigated dynamics intracellular distribution living rat culture transfected with a genetically encoded sensor for cGMP. We demonstrate that treated pharmacological stimuli able activate membrane soluble guanylyl cyclase (sGC) presented an increase entire neuron, from cilia-dendrite axon terminus-growth cone. Upon stimulation, rise was again found including terminus, where it locally synthesized. odorant-dependent due sGC activation by nitric oxide (NO) requires cAMP. link between cAMP NO synthase appears be cytosolic Ca<sup>2+</sup> concentration elicited either plasma channel mobilization stores via guanine nucleotide exchange factor Epac. Finally, show can elicit both <i>in vitro</i> vivo</i> phosphorylation nuclear CREB, suggesting signaling pathway may relevant local events (pathfinding, neurotransmitter release) more distal involving gene expression regulation.
SUPPLEMENTAL MATERIAL
Coming soon ....
REFERENCES (0)
CITATIONS (35)
EXTERNAL LINKS
PlumX Metrics
RECOMMENDATIONS
FAIR ASSESSMENT
Coming soon ....
JUPYTER LAB
Coming soon ....