HSF 1 deficiency and impaired HSP 90‐dependent protein folding are hallmarks of aneuploid human cells

• DOI: 10.15252/embj.201488648
• Publication Date: 2014-09-10T05:23:54Z
• Authors: Neysan Donnelly; Verena Passerini; Milena Dürrbaum; Silvia Stingele; Zuzana Storchová
• Keywords: 0301 basic medicine; Proteasome Endopeptidase Complex; Protein Folding; Proteome; Cell Survival; Gene Expression; Aneuploidy; Cell Line; DNA-Binding Proteins; 03 medical and health sciences; Phenotype; Gene Expression Regulation; Heat Shock Transcription Factors; Mutation; Humans; HSP90 Heat-Shock Proteins; Proteostasis Deficiencies; Promoter Regions, Genetic; Transcriptome; Heat-Shock Response; Transcription Factors

Aneuploidy is a hallmark of cancer and is associated with malignancy and poor prognosis. Recent studies have revealed that aneuploidy inhibits proliferation, causes distinct alterations in the transcriptome and proteome and disturbs cellular proteostasis. However, the molecular mechanisms underlying the changes in gene expression and the impairment of proteostasis are not understood. Here, we report that human aneuploid cells are impaired in HSP90-mediated protein folding. We show that aneuploidy impairs induction of the heat shock response suggesting that the activity of the transcription factor heat shock factor 1 (HSF1) is compromised. Indeed, increased levels of HSF1 counteract the effects of aneuploidy on HSP90 expression and protein folding, identifying HSF1 overexpression as the first aneuploidy-tolerating mutation in human cells. Thus, impaired HSF1 activity emerges as a critical factor underlying the phenotypes linked to aneuploidy. Finally, we demonstrate that deficient protein folding capacity directly shapes gene expression in aneuploid cells. Our study provides mechanistic insight into the causes of the disturbed proteostasis in aneuploids and deepens our understanding of the role of HSF1 in cytoprotection and carcinogenesis.