STARD3 mediates endoplasmic reticulum‐to‐endosome cholesterol transport at membrane contact sites
membrane contact site
0303 health sciences
Cell Membrane
Vesicular Transport Proteins
cholesterol
Membrane Proteins
Biological Transport
lipid transfer protein
[SDV.BC.BC]Life Sciences [q-bio]/Cellular Biology/Subcellular Processes [q-bio.SC]
Articles
Endosomes
Endoplasmic Reticulum
endoplasmic reticulum
03 medical and health sciences
Cholesterol
Humans
Carrier Proteins
endosome
HeLa Cells
Protein Binding
DOI:
10.15252/embj.201695917
Publication Date:
2017-04-05T00:18:48Z
AUTHORS (8)
ABSTRACT
AbstractStAR‐related lipid transfer domain‐3 (STARD3) is a sterol‐binding protein that creates endoplasmic reticulum (ER)–endosome contact sites. How this protein, at the crossroad between sterol uptake and synthesis pathways, impacts the intracellular distribution of this lipid was ill‐defined. Here, by using in situ cholesterol labeling and quantification, we demonstrated that STARD3 induces cholesterol accumulation in endosomes at the expense of the plasma membrane. STARD3‐mediated cholesterol routing depends both on its lipid transfer activity and its ability to create ER–endosome contacts. Corroborating this, in vitro reconstitution assays indicated that STARD3 and its ER‐anchored partner, Vesicle‐associated membrane protein‐associated protein (VAP), assemble into a machine that allows a highly efficient transport of cholesterol within membrane contacts. Thus, STARD3 is a cholesterol transporter scaffolding ER–endosome contacts and modulating cellular cholesterol repartition by delivering cholesterol to endosomes.
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