Cohesins and condensins orchestrate the 4D dynamics of yeast chromosomes during the cell cycle

• DOI: 10.15252/embj.201797342
• Publication Date: 2017-07-21T00:25:31Z
• Authors: Luciana Lazar‐Ste...; Vittore F Scolari; Guillaume Mercy; Héloise Muller; Thomas M Guérin; Agnès Thierry; Julien Mozziconacci; Romain Koszul
• Keywords: Condensin; Schizosaccharomyces

Duplication and segregation of chromosomes involves dynamic reorganization their internal structure by conserved architectural proteins, including the structural maintenance (SMC) complexes cohesin condensin. Despite active investigation roles these factors, a genome-wide view chromosome architecture at both small large scale during cell division is still missing. Here, we report first comprehensive 4D analysis higher-order organization Saccharomyces cerevisiae genome throughout cycle investigate SMC in controlling transitions. During replication, cohesion establishment promotes numerous long-range intra-chromosomal contacts correlates with individualization chromosomes, which culminates metaphase. In anaphase, mitotic are abruptly reorganized depending on mechanical forces exerted spindle. Formation condensin-dependent loop bridging centromere cluster rDNA loci suggests that condensin-mediated may also directly facilitate segregation. This work therefore comprehensively recapitulates cycle-dependent dynamics unicellular eukaryote, but unveils new features highly stages division.