The glycine arginine‐rich domain of the RNA‐binding protein nucleolin regulates its subcellular localization

• DOI: 10.15252/embj.2020107158
• Publication Date: 2021-09-13T10:51:20Z
• Authors: Ella Doron‐Mandel; Indrek Koppel; Ofri Abraham; Ida Rishal; Terika P Smith; Courtney N Buchanan; Pabitra K Sahoo; Jan Kadlec; Juan A Oses‐Prieto; Riki Kawaguchi; Stefanie Alber; Eitan Erez Zahavi; Pierluigi Di Matteo; Agostina Di Pizio; Didi‐Andreas Song; Nataliya Okladnikov; Dalia Gordon; Shifra Ben‐Dor; Rebecca Haffner‐K...; Giovanni Coppola; Alma L Burlingame; Pavel Jungwirth; Jeffery L Twiss; Mike Fainzilber
• Keywords: Male; Neurons; 0301 basic medicine; Mice, Inbred BALB C; Primary Cell Culture; Gene Expression; Kinesins; Articles; Phosphoproteins; Axonal Transport; Mice, Inbred C57BL; Mice; 03 medical and health sciences; HEK293 Cells; Protein Domains; Cell Line, Tumor; Ganglia, Spinal; Mutation; Animals; Humans; Amino Acid Sequence; Cell Nucleolus; HeLa Cells

Nucleolin is a multifunctional RNA Binding Protein (RBP) with diverse subcellular localizations, including the nucleolus in all eukaryotic cells, the plasma membrane in tumor cells, and the axon in neurons. Here we show that the glycine arginine rich (GAR) domain of nucleolin drives subcellular localization via protein-protein interactions with a kinesin light chain. In addition, GAR sequences mediate plasma membrane interactions of nucleolin. Both these modalities are in addition to the already reported involvement of the GAR domain in liquid-liquid phase separation in the nucleolus. Nucleolin transport to axons requires the GAR domain, and heterozygous GAR deletion mice reveal reduced axonal localization of nucleolin cargo mRNAs and enhanced sensory neuron growth. Thus, the GAR domain governs axonal transport of a growth controlling RNA-RBP complex in neurons, and is a versatile localization determinant for different subcellular compartments. Localization determination by GAR domains may explain why GAR mutants in diverse RBPs are associated with neurodegenerative disease.