Ablation of mitochondrial DNA results in widespread remodeling of the mitochondrial complexome

• DOI: 10.15252/embj.2021108648
• Publication Date: 2021-09-20T13:40:48Z
• Authors: Sergio Guerrero‐C...; Joeri van Strien; Ulrich Brandt; Susanne Arnold
• Keywords: DNAJA3

So-called ρ0 cells lack mitochondrial DNA and are therefore incapable of aerobic ATP synthesis. How adapt to survive ablation oxidative phosphorylation remains poorly understood. Complexome profiling analysis covered 1,002 proteins revealed changes in abundance organization numerous multiprotein complexes including previously not described assemblies. Beyond multiple subassemblies that would normally contain components encoded by DNA, we observed widespread reorganization the complexome. This included distinct expression pattern adenine nucleotide carrier isoforms, other transporters, protein import machinery. Remarkably, hardly affected organizing cristae junctions indicating altered morphology mitochondria predominantly resulted from loss complex V dimers required impose narrow curvatures inner membrane. Our data provide a comprehensive resource for in-depth remodeling complexome response respiratory deficiency.