The Hda1 histone deacetylase limits divergent non‐coding transcription and restricts transcription initiation frequency
RNA polymerase II
General transcription factor
Transcription
E-box
Transcription coregulator
Response element
DOI:
10.15252/embj.2021108903
Publication Date:
2021-10-21T07:05:01Z
AUTHORS (8)
ABSTRACT
Nucleosome-depleted regions (NDRs) at gene promoters support initiation of RNA polymerase II transcription. Interestingly, transcription often initiates in both directions, resulting an mRNA and a divergent non-coding (DNC) transcript unclear purpose. Here, we characterized the genetic architecture molecular mechanism DNC budding yeast. Using high-throughput reverse screens based on quantitative single-cell fluorescence measurements, identified Hda1 histone deacetylase complex (Hda1C) as repressor Nascent profiling showed genome-wide role Hda1C repression Live-cell imaging revealed that mutations Hda3 subunit increased frequency contributed to decreased acetylation H3 regions, supporting by deacetylation. Our data interpretation results consequence NDR-based eukaryotic promoters, but it is governed locus-specific maintain genome fidelity.
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