The Hda1 histone deacetylase limits divergent non‐coding transcription and restricts transcription initiation frequency

• DOI: 10.15252/embj.2021108903
• Publication Date: 2021-10-21T07:05:01Z
• Authors: Uthra Gowthaman; Maxim Ivanov; Isabel Schwarz; Heta P Patel; Niels A Müller; Desiré García‐Pic...; Tineke L Lenstra; Sebastian Marquardt
• Keywords: RNA polymerase II; General transcription factor; Transcription; E-box; Transcription coregulator; Response element

Nucleosome-depleted regions (NDRs) at gene promoters support initiation of RNA polymerase II transcription. Interestingly, transcription often initiates in both directions, resulting an mRNA and a divergent non-coding (DNC) transcript unclear purpose. Here, we characterized the genetic architecture molecular mechanism DNC budding yeast. Using high-throughput reverse screens based on quantitative single-cell fluorescence measurements, identified Hda1 histone deacetylase complex (Hda1C) as repressor Nascent profiling showed genome-wide role Hda1C repression Live-cell imaging revealed that mutations Hda3 subunit increased frequency contributed to decreased acetylation H3 regions, supporting by deacetylation. Our data interpretation results consequence NDR-based eukaryotic promoters, but it is governed locus-specific maintain genome fidelity.