Gene‐specific correlation of RNA and protein levels in human cells and tissues

• DOI: 10.15252/msb.20167144
• Publication Date: 2016-10-21T00:58:22Z
• Authors: Fredrik Edfors; Frida Danielsson; Björn M Hallström; Lukas Käll; Emma Lundberg; Fredrik Pontén; Björn Forsström; Mathias Uhlén
• Keywords: Proteomics; Medicin och hälsovetenskap; targeted proteomics; Medicine (General); protein quantification; Proteome; QH301-705.5; Gene Expression Profiling; Gene Expression; Protein quantification; Articles; Medical and Health Sciences; Cell Line; transcriptomics; R5-920; gene expression; Humans; Gene expression; Biology (General); Transcriptomics; Targeted proteomics

An important issue for molecular biology is to establish whether transcript levels of a given gene can be used as proxies the corresponding protein levels. Here, we have developed targeted proteomics approach set human non-secreted proteins based on parallel reaction monitoring measure, at steady-state conditions, absolute copy numbers across tissues and cell lines compared these with mRNA using transcriptomics. The study shows that do not correlate well unless gene-specific RNA-to-protein (RTP) conversion factor independent tissue type introduced, thus significantly enhancing predictability from RNA results show RTP ratio varies few hundred copies per molecule some genes several thousands others. In conclusion, our data suggest transcriptome analysis tool predict cell, forming an attractive link between field genomics proteomics.