Transcriptional Signature of Valproic Acid-Induced Neural Tube Defects in Human Spinal Cord Organoids

• DOI: 10.15283/ijsc23012
• Publication Date: 2023-08-30T00:02:43Z
• Authors: Ju-Hyun Lee; Mohammed R. Shaker; Si-Hyung Park; Woong Sun
• Keywords: Neurulation; Organoid; Model Organism; Neural tube defect

In vertebrates, the entire central nervous system is derived from neural tube, which formed through a conserved early developmental morphogenetic process called neurulation. Although perturbations in neurulation caused by genetic or environmental factors lead to tube defects (NTDs), most common congenital malformation and precise molecular pathological cascades mediating NTDs are not well understood. Recently, we have developed human spinal cord organoids (hSCOs) that recapitulate some aspects of observed valproic acid (VPA) could cause an organoid model. this study, identified verified significant changes cell-cell junctional genes/proteins VPA-treated using transcriptomic immunostaining analysis. Furthermore, mouse embryos exhibited impaired gene expression NTD phenotypes, similar those hSCO Collectively, our data demonstrate hSCOs provide valuable biological resource for dissecting pathways underlying currently unknown destructive analysis tools.