Effects of follicular fluid at fertilization in vitro on sperm penetration in pig oocytes

• DOI: 10.1530/jrf.0.0990097
• Publication Date: 2004-03-20T06:55:52Z
• Authors: H. Funahashi; B. N. Day
• Keywords: Follicular fluid; Penetration (warfare)

The effects of porcine follicular fluid (PFF) on sperm penetration pig oocytes and prevention polyspermy were examined characteristics spermatozoa exposed to PFF determined. addition at the level 1 10% prefertilization fertilization media decreased rates mean number in penetrated eggs regardless origin PFF. In presence BSA, supplementation 0.1% 1% did not decrease but increase monospermic 54 68%, respectively. When was added media, binding zona percentage acrosome-intact with increased concentration (from 43.1 ± 2.8 73.1 4.9% 7.2 1.3 15.7 15.4%, respectively). At end incubation, agglutination observed degree depended concentration. Supplementation fetal calf serum blocked zona. These results indicate that incubation suitable concentrations will effectively reduce both attach surface incidence polyspermy.