Piezo-assisted nuclear transfer affects cloning efficiency and may cause apoptosis

• DOI: 10.1530/rep-06-0358
• Publication Date: 2007-07-06T18:52:55Z
• Authors: Yang Yu; Chenhui Ding; Eryao Wang; Xinjie Chen; Xuemei Li; Chunli Zhao; Yong Fan; Liu Wang; Nathalie Beaujean; Qi Zhou; Alice Jouneau; Weizhi Ji
• Keywords: Electrofusion; Blastomere; Cloning (programming); Reprogramming

Even though it generates healthy adults, nuclear transfer in mammals remains an inefficient process. Mainly attributed to abnormal reprograming of the donor chromatin, this inefficiency may also be caused at least partly by a specific effect cloning technique which has not yet been well investigated. There are two main procedures for transferring nuclei into enucleated oocytes: fusion and piezoelectric microinjection, latter being used mostly mice. We have, therefore, decided compare quality developmental ability, both vivo vitro , embryos reconstructed with electrofusion or injection. In addition, piezo setups differing electric strengths was Along record rate development, we compared integrity blastomeres during first cleavages as morphological cellular blastocysts. Our results show that piezo-assisted micromanipulation can induce DNA damage embryos, apoptosis, reduced cell numbers blastocysts lower development term. if piezo-driven injection facilitates faster more efficient reconstruction, should precaution low parameters possible.