Differences in the transcriptional profiles of human cumulus cells isolated from MI and MII oocytes of patients with polycystic ovary syndrome

• DOI: 10.1530/rep-13-0005
• Publication Date: 2013-04-20T02:45:19Z
• Authors: Xin Huang; Cuifang Hao; Xiaofang Shen; Xiaoyan Liu; Yinghua Shan; Yuhua Zhang; Lili Chen
• Keywords: Adult; Cumulus Cells; Transcription, Genetic; Reverse Transcriptase Polymerase Chain Reaction; Gene Expression Profiling; Embryonic Development; Fertilization in Vitro; Embryo Transfer; In Vitro Oocyte Maturation Techniques; 3. Good health; Cohort Studies; 03 medical and health sciences; Oogenesis; 0302 clinical medicine; Ovulation Induction; Pregnancy; Oocytes; Humans; Female; Biomarkers; Cells, Cultured; Oligonucleotide Array Sequence Analysis; Polycystic Ovary Syndrome

Polycystic ovary syndrome (PCOS) is a common endocrine and metabolic disorder in women. The abnormalities of intra-ovarian paracrine interactions may change the microenvironment for oocyte development during folliculogenesis process reduce developmental competence oocytes PCOS patients who are suffering from anovulatory infertility pregnancy loss. In this microenvironment, cross talk between an surrounding cumulus cells (CCs) critical achieving competence. aim our study was to investigate gene expression profiles CCs obtained undergoing IVF cycles terms maturation by using human Genome U133 Plus 2.0 microarrays. A total 59 genes were differentially expressed two CC groups. Most these identified be involved one or more following pathways: receptor interactions, calcium signaling, metabolism biosynthesis, focal adhesion, melanogenesis, leukocyte transendothelial migration, Wnt type 2 diabetes mellitus. According different levels microarrays their putative functions, six (LHCGR, ANGPTL1, TNIK, GRIN2A, SFRP4, SOCS3) selected analyzed quantitative RT-PCR (qRT-PCR). qRT-PCR results consistent with microarray data. Moreover, molecular signatures associated potential embryo blastocyst stage proposed as biomarkers viability patients. Our clinically important they offer new strategy competent oocyte/embryo selection