SRC family kinases in hamster spermatozoa: evidence for the presence of LCK

• DOI: 10.1530/rep-16-0591
• Publication Date: 2017-03-02T01:20:23Z
• Authors: Durgesh Kumar Singh; Rohit Kumar Deshmukh; Praveen Kumar Nar...; Sisinthy Shivaji; Archana Bharadwaj...
• Keywords: Capacitation; Hyperactivation; Acrosome reaction; Src family kinase

Sperm capacitation is a prerequisite for successful fertilization. Increase in tyrosine phosphorylation considered the hallmark of and attempts to understand its regulation are ongoing. In this regard, we attempted study role SRC family kinases (SFKs) hamster sperm functions. Interestingly, found presence lymphocyte-specific protein kinase, LCK, mammalian spermatozoa further characterized it terms localization function. LCK was several species, transcript identified testis. Autophosphorylation at Y394 residue increased as progressed, indicating an upregulation activity during capacitation. Inhibition (and perhaps other SFKs) with use specific inhibitor showed significant decrease proteins, implying LCK/SFKs key kinase(s) regulating Dihydrolipoamide dehydrogenase substrate LCK/SFK. inhibition significantly reduced percentage fertilization (in vitro) but had no effect on motility, hyperactivation acrosome reaction. summary, first report SFK hematopoietic lineage besides being SFKs Syrian hamsters.