SRC family kinases in hamster spermatozoa: evidence for the presence of LCK
Male
0301 basic medicine
0303 health sciences
Mesocricetus
Acrosome Reaction
Fertilization in Vitro
Spermatozoa
03 medical and health sciences
src-Family Kinases
Lymphocyte Specific Protein Tyrosine Kinase p56(lck)
Cricetinae
Testis
Cyclic AMP
Oocytes
Sperm Motility
Animals
Humans
Tyrosine
Female
Phosphorylation
Sperm Capacitation
Cells, Cultured
DOI:
10.1530/rep-16-0591
Publication Date:
2017-03-02T01:20:23Z
AUTHORS (5)
ABSTRACT
Sperm capacitation is a prerequisite for successful fertilization. Increase in tyrosine phosphorylation is considered the hallmark of capacitation and attempts to understand its regulation are ongoing. In this regard, we attempted to study the role of SRC family kinases (SFKs) in the hamster sperm functions. Interestingly, we found the presence of the lymphocyte-specific protein tyrosine kinase, LCK, in mammalian spermatozoa and further characterized it in terms of its localization and function. LCK was found in spermatozoa of several species, and its transcript was identified in the hamster testis. Autophosphorylation of LCK at the Y394 residue increased as capacitation progressed, indicating an upregulation of LCK activity during capacitation. Inhibition of LCK (and perhaps the other SFKs) with the use of a specific inhibitor showed a significant decrease in protein tyrosine phosphorylation of several proteins, implying LCK/SFKs as key tyrosine kinase(s) regulating tyrosine phosphorylation during hamster sperm capacitation. Dihydrolipoamide dehydrogenase was identified as a substrate for LCK/SFK. LCK/SFKs inhibition significantly reduced the percentage fertilization (in vitro) but had no effect on sperm motility, hyperactivation and acrosome reaction. In summary, this is the first report on the presence of LCK, an SFK of hematopoietic lineage in spermatozoa besides being the first study on the role of SFKs in the spermatozoa of Syrian hamsters.
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