SRC family kinases in hamster spermatozoa: evidence for the presence of LCK

Male 0301 basic medicine 0303 health sciences Mesocricetus Acrosome Reaction Fertilization in Vitro Spermatozoa 03 medical and health sciences src-Family Kinases Lymphocyte Specific Protein Tyrosine Kinase p56(lck) Cricetinae Testis Cyclic AMP Oocytes Sperm Motility Animals Humans Tyrosine Female Phosphorylation Sperm Capacitation Cells, Cultured
DOI: 10.1530/rep-16-0591 Publication Date: 2017-03-02T01:20:23Z
ABSTRACT
Sperm capacitation is a prerequisite for successful fertilization. Increase in tyrosine phosphorylation is considered the hallmark of capacitation and attempts to understand its regulation are ongoing. In this regard, we attempted to study the role of SRC family kinases (SFKs) in the hamster sperm functions. Interestingly, we found the presence of the lymphocyte-specific protein tyrosine kinase, LCK, in mammalian spermatozoa and further characterized it in terms of its localization and function. LCK was found in spermatozoa of several species, and its transcript was identified in the hamster testis. Autophosphorylation of LCK at the Y394 residue increased as capacitation progressed, indicating an upregulation of LCK activity during capacitation. Inhibition of LCK (and perhaps the other SFKs) with the use of a specific inhibitor showed a significant decrease in protein tyrosine phosphorylation of several proteins, implying LCK/SFKs as key tyrosine kinase(s) regulating tyrosine phosphorylation during hamster sperm capacitation. Dihydrolipoamide dehydrogenase was identified as a substrate for LCK/SFK. LCK/SFKs inhibition significantly reduced the percentage fertilization (in vitro) but had no effect on sperm motility, hyperactivation and acrosome reaction. In summary, this is the first report on the presence of LCK, an SFK of hematopoietic lineage in spermatozoa besides being the first study on the role of SFKs in the spermatozoa of Syrian hamsters.
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