The interaction between seminal plasma, sperm, and endometrium in inter- and intra-species breeding in equids

• DOI: 10.1530/rep-23-0472
• Publication Date: 2024-04-05T16:33:55Z
• Authors: Giorgia Podico; João H Bittar; Shavahn C Loux; Fabiana F Souza; Igor F Canisso
• Keywords: Endometritis

In brief some instances, extra-species breeding in equids is more successful than intraspecies breeding; however, little known about the immunomodulatory effect of donkey semen and seminal plasma on mare’s endometrium. This study compared mare uterine inflammatory response during extra- breeding. Abstract Anecdotal experience suggests horse mares have less post-breeding inflammation better fertility when bred with donkeys. aimed to compare exposed evaluate proteome metabolome sperm plasma. Uterine edema, intrauterine fluid accumulation, polymorphonuclear neutrophils cytology, concentrations progesterone, pro- anti-inflammatory cytokines (IL1A, IL1B, IL4, IL6, CXCL8, IL10) were assessed pre- post infusion (donkey horse). The analyzed by LC-MS/MS. Mare cycles had a greater progesterone concentration those at 8 days ovulation ( P = 0.046). At 6 h infusion, due tended be lower 0.074). Donkey properties plasma, as determined fewer cytology < 0.05). Horse resulted IL6 lesser IL1B PGE1, PGE3, lactoferrin significantly abundant Prostaglandins play an important role immunomodulation might contribute triggered interspecies conclusion, induces similar endometritis observed semen. results post-infusion other combinations immediate post-breeding.