The Origin Recognition Complex Links Replication, Sister Chromatid Cohesion and Transcriptional Silencing in Saccharomyces cerevisiae

• DOI: 10.1534/genetics.103.024851
• Publication Date: 2004-08-02T23:56:34Z
• Authors: Bernhard Suter; Amy Tong; Michael Chang; Lisa Yu; Grant W Brown; Charles Boone; Jasper Rine
• Keywords: Origin recognition complex

Abstract Mutations in genes encoding the origin recognition complex (ORC) of Saccharomyces cerevisiae affect initiation DNA replication and transcriptional repression at silent mating-type loci. To explore function ORC more detail, a screen for genetic interactions was undertaken using large-scale synthetic lethal analysis. Combination orc2-1 orc5-1 alleles with complete set haploid deletion mutants revealed lethal/sick phenotypes involved replication, chromatin structure, checkpoints, repair recombination, other that were unexpected on basis previous studies ORC. Many these are shared replication. Strong demonstrated null mutations contribute to sister chromatid cohesion. A interaction between cohesin mutant scc1-73 suggested contributes Thus, comprehensive screening gene connection Further experiments linked cohesion silencing loci telomeres.