Interaction of Epe1 With the Heterochromatin Assembly Pathway inSchizosaccharomyces pombe
0303 health sciences
Chromosomal Proteins, Non-Histone
Recombinant Fusion Proteins
Genes, Fungal
Nuclear Proteins
Chromatin Assembly and Disassembly
Methylation
Histone Deacetylases
Histones
Luminescent Proteins
03 medical and health sciences
Bacterial Proteins
Microscopy, Fluorescence
Gene Expression Regulation, Fungal
Heterochromatin
Mutation
Schizosaccharomyces
Gene Silencing
Schizosaccharomyces pombe Proteins
Promoter Regions, Genetic
DOI:
10.1534/genetics.106.068684
Publication Date:
2007-04-20T23:43:25Z
AUTHORS (7)
ABSTRACT
AbstractEpe1 is a JmjC domain protein that antagonizes heterochromatization in Schizosaccharomyces pombe. Related JmjC domain proteins catalyze a histone demethylation reaction that depends on Fe(II) and α-ketoglutarate. However, no detectable demethylase activity is associated with Epe1, and its JmjC domain lacks conservation of Fe(II)-binding residues. We report that Swi6 recruits Epe1 to heterochromatin and that overexpression of epe1+, like mutations in silencing genes or overexpression of swi6+, upregulates expression of certain genes. A significant overlap was observed between the lists of genes that are upregulated by overexpression of epe1+ and those that are upregulated by mutations in histone deacetylase genes. However, most of the common genes are not regulated by Clr4 histone methyltransferase. This suggests that Epe1 interacts with the heterochromatin assembly pathway at the stage of histone deacetylation. Mutational inactivation of Epe1 downregulates ∼12% of S. pombe genes, and the list of these genes overlaps significantly with the lists of genes that are upregulated by mutations in silencing genes and genes that are hyperacetylated at their promoter regions in clr6-1 mutants. We propose that an interplay between the repressive HDACs activity and Epe1 helps to regulate gene expression in S. pombe.
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