Identification of Small RNAs Associated with Meiotic Silencing by Unpaired DNA

• DOI: 10.1534/genetics.112.149138
• Publication Date: 2013-03-16T08:27:46Z
• Authors: Thomas M Hammond; William G Spollen; Logan M Decker; Sean M Blake; Gordon K Springer; Patrick K T Shiu
• Keywords: 0303 health sciences; Base Sequence; Neurospora crassa; Nucleotides; Genes, Fungal; Reproducibility of Results; RNA, Fungal; Exons; Spores, Fungal; Introns; GC Rich Sequence; Meiosis; 03 medical and health sciences; Genetic Loci; Gene Silencing; RNA, Small Interfering; DNA, Fungal; Crosses, Genetic

AbstractIn Neurospora crassa, unpaired genes are silenced by a mechanism called meiotic silencing by unpaired DNA (MSUD). Although some RNA interference proteins are necessary for this process, its requirement of small RNAs has yet to be formally established. Here we report the characterization of small RNAs targeting an unpaired region, using Illumina sequencing.