Linkage Disequilibrium with Linkage Analysis of Multiline Crosses Reveals Different Multiallelic QTL for Hybrid Performance in the Flint and Dent Heterotic Groups of Maize

• DOI: 10.1534/genetics.114.169367
• Publication Date: 2014-10-01T00:13:59Z
• Authors: Héloïse Giraud; Christina Lehermeier; Eva Bauer; Matthieu Falque; Vincent Segura; Cyril Bauland; Christian Camisan; Laura Campo; Nina Meyer; Nicolas Ranc; Wolfgang Schipprack; Pascal Flament; Albrecht E Melchi...; Monica Menz; Jesús Moreno-Gonz...; Milena Ouzunova; Alain Charcosset; Chris-Carolin Schön; Laurence Moreau
• Keywords: Linkage Disequilibrium; Family-based QTL mapping; Association mapping; Genetic linkage; Linkage (software)

Abstract Multiparental designs combined with dense genotyping of parents have been proposed as a way to increase the diversity and resolution quantitative trait loci (QTL) mapping studies, using methods combining linkage disequilibrium information analysis (LDLA). Two new nested association adapted European conditions were derived from complementary dent flint heterotic groups maize (Zea mays L.). Ten biparental families (N = 841) 11 811) genotyped 56,110 single nucleotide polymorphism markers evaluated test crosses central line reciprocal design for biomass yield, plant height, precocity. Alleles at candidate QTL defined (i) parental alleles, (ii) haplotypic identity by descent, (iii) single-marker groupings. Between five 16 detected depending on model, trait, genetic group considered. In design, major (R2 27%) pleiotropic effects was chromosome 10, whereas other displayed milder < 10%). On average, LDLA models more but generally explained lower percentages variance, consistent fact that most display complex allelic series. Only 15% common two designs. A joint between 15 21 traits. Of these, 27 silking date 41% tasseling significant in both groups. Favorable open perspectives improving production.