Detection of Viral RNA by Electron Microscopic In situ Hybridization(ISH-EM) in The Germinal Epithelium of Mice Infected with Encephalomyocarditis(EMC) Virus.

• DOI: 10.1538/expanim.46.79
• Publication Date: 2004-01-14T09:51:19Z
• Authors: Aito UENO; Makio TAKEDA; Kensuke HIRASAWA; Shin-ichi ITAGAKI; Kunio DOI
• Keywords: Germinal epithelium

Electron microscopic in situ hybridization (ISH-EM) was first applied to the detection of viral RNA germinal epithelium mice inoculated i.p. with 10(5) plaque-forming units/mouse D variant encephalomyocarditis virus (EMC-D). Signals were detected a small number Sertoli cells showing mild degeneration at 2 days post inoculation, and later, they also spermatogonia when showed prominent degeneration. The results clearly demonstrated that site attack cell case EMC-D-induced mouse orchitis.