Evaluation of PCR as a Means of Identification of Pasteurella pneumotropica.

• DOI: 10.1538/expanim.48.51
• Publication Date: 2004-01-14T09:51:19Z
• Authors: Ryoko NOZU; Kazuo GOTO; Hiroaki OHASHI; Akira TAKAKURA; Toshio ITOH
• Keywords: DNA, Bacterial; 0301 basic medicine; Base Sequence; Molecular Sequence Data; Pasteurella Infections; Reproducibility of Results; DNA, Ribosomal; Polymerase Chain Reaction; Sensitivity and Specificity; 3. Good health; 03 medical and health sciences; Animals, Laboratory; RNA, Ribosomal, 16S; Animals; Pasteurella

A polymerase chain reaction with new primers (new PCR) designed from Pasteurella pneumotropica 16S rDNA as an identification system for this organism was compared the PCR reported by Wang et al. (Wang's using 15 bacterial reference species and 70 clinical isolates conventional system. For strains, both PCRs were identical. isolates, Wang's showed consistency in 62.9% (44/70) 51.4% (36/70), respectively. Twenty-six inconsistent respect to morphology serology. These findings suggested that more sensitive than PCR, combination serology is useful P. identification.