Comparison of Bacteriological, Genetic and Pathological Characters between Escherichia coli O115a,c:K(B) and Citrobacter rodentium.

DNA, Bacterial Male 0301 basic medicine Mice, Inbred BALB C 0303 health sciences Hyperplasia Colon Sequence Homology Sequence Analysis, DNA 3. Good health Citrobacter freundii Rodent Diseases Colonic Diseases Mice 03 medical and health sciences RNA, Ribosomal, 16S Escherichia coli Animals Germ-Free Life Female Disease Susceptibility
DOI: 10.1538/expanim.50.183 Publication Date: 2004-01-14T09:54:10Z
ABSTRACT
Murine pathogenic Escherichia coli O115a,c:K(B) (MPEC) is the causative agent of mouse megaenteron, the pathology of which resembles that of transmissible murine colonic hyperplasia caused by Citrobacter rodentium. We compared their genetic and pathological features to reveal the relationship between these two bacteria. To evaluate the genetic distances, 16S rDNA genes were sequenced and biochemical reactions were tested. Mouse strain susceptibility tests, using CF1 MPEC-susceptible germfree mice and BALB/cA(Jic) resistant mice were performed. MPEC strains and C. rodentium showed more than 99.6% identity by comparison of 16S rDNA gene sequences. All results from biochemical reactions and the mouse strain susceptibility tests were identical. It is proposed that MPEC should be reclassified as C. rodentium.
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