Effect of nicotinamide on amino acids content in bone collagen depending on biological availability of vitamins in diabetic rats

• DOI: 10.15407/ubj86.04.138
• Publication Date: 2015-07-30T13:39:06Z
• Authors: M. M. Guzyk; Iu. T. Sergiichuk; ; ; K. O. Dyakun; ; ; L. V. Yanitska; ; T. M. Kuchmerovska
• Keywords: Hydroxyproline

Connective tissue is highly susceptible to imbalances induced by diabetes. Diabetes-related osteopenia, decreased bone strength etc. may be associated with altered metabolism of various collagens: Although it assumed that alterations in collagen amino acids (AA) strongly affect protein properties andphysiological functions, however, very limited evidences are present at the moment regarding AA composition type I and its relevance abnormal availability vitamins which necessary for synthesis We have tested whether nicotinamide (NAm) can influence Icollagen formation as well After 4 weeks STZ-induced diabetes (60 mg/ kg) male Wistar rats were injected 2 with/without NAm (200 mg/kg b. w). Acid extraction from bones was performed following stepwise salting out. The content after acid estimated amounts hydroxyproline. Amino assayed cation exchange chromatography Diabetes-associated changes mainly those known involved helix cross-linking molecules. Diabetes found significantly reduce contents o-Pro, Gly, Ala, o-Lys Pro, whereas Lys, His, Arg, Glu, Thr, Leu, Phe elevated (P < 0.05). treatment able partially normalise contents. In diabetes, blood serum hepatic vitamin C B3 shown lowered, a-tocopherol slightly increased compared control Restoration circulatory liver observed. data demonstrate close relationship between diabetes-associated decrease deposition, impaired vitamins, synthesis. Thus, might a useful agent failures related