ANTIOXIDANT ENZYMES ACTIVITY IN EMBRYOGENIC AND NON-EMBRYOGENIC TISSUES IN SUGARCANE

• DOI: 10.15446/abc.v19n2.39200
• Publication Date: 2014-06-16T17:14:27Z
• Authors: Marina Medeiros d...; Cláudia Ulisses; Maria Jaislanny L...; Manuela Maria Cav...; Lilia Willadino; Terezinha Camara
• Keywords: embriogénesis somática; 0301 basic medicine; QH301-705.5; Saccharum officinarum L.; 2; ácido 2; somatic embryogenesis; 4-D); 4-diclorofenoxiacético (2; 03 medical and health sciences; oxidative stress; Cultivo de tejidos; 4-dichlorophenoxyacetic acid (2; Biology (General); Ciencias Biologicas; 2 4-dichlorophenoxyacetic acid (2 4-D)

The objective of this work was to induce direct somatic embryogenesis from segments immature leaves the RB872552 variety sugarcane and correlate morphogenic event with oxidative stress. Two previously described protocols were utilized for induction in different supplementations culture medium incubation conditions. For conversion embryos into plants used MS without phytoregulators. Histological analyses activity antioxidant enzymes also conducted embryogenic non-embryogenic tissues. formation obtained 81 % explants combination regulators 2,4-D (2,4-dichlorophenoxyacetic acid)and BAP (6-benzylaminopurine) when incubated under 16 h photoperiod. With regards enzymes, there increased peroxidase an increase soluble protein content tissues, whereas lower activities polyphenol oxidase catalase appeared these tissues compared nonembryogenic It could be inferred that stress plays important role sugarcane.