Prevalence of carbapenemase genes among multidrug-resistant Pseudomonas aeruginosa isolates from tertiary care centers in Southern Thailand

• DOI: 10.15537/smj.2022.43.9.20220219
• Publication Date: 2022-09-14T09:10:17Z
• Authors: Phanvasri Saengsuwan; Narongdet Kositpa...; Soontara Kawila; Wichien Patugkaro; Chonticha Romyasamit
• Keywords: Tertiary care

To assess the prevalence of carbapenemase genes among multidrug-resistant Pseudomonas aeruginosa (P. aeruginosa) isolates from tertiary care centers in Southern Thailand.The P. collected patients hospitalized between 2015-2017 2 hospitals Songkhla Province, Thailand, was investigated. Standard laboratory procedures were followed and disk diffusion test used for bacterial identification susceptibility evaluations. Carbapenemase detected using multiplex polymerase chain reaction (PCR) genotyping by pulsed field gel electrophoresis.Among 289 isolates, 55% sputum, 19.4% urine, 8% secretions. The 55.7% carbapenem-resistant (CR-MDR-PA) 39.4% (MDR-PA). Resistance to imipenem, meropenem, gentamicin, ceftazidime ranged 50-60%, amikacin most effective antibiotic (38.4%). blaVIM (27.7%), blaIMP (23.9%), blaOXA48 (4.8%) detected; however, blaSPM blaBIC not any isolates. Pulsed electrophoresis analysis revealed clonal diversity 17 CR-MDR-PA strains.A high percentage carries our area; therefore, more emphasis on application molecular techniques infection prevention control may provide useful insights disease epidemiology.