Resistome analysis of Escherichia coli isolates from layers in Hungary

• DOI: 10.1556/004.2024.00988
• Publication Date: 2024-04-05T11:53:22Z
• Authors: Ádám Kerek; Ábel Szabó; Krisztián Bányai; Eszter Kaszab; Krisztina Bali; Márton Papp; László Kovács; Ákos Jerzsele
• Keywords: 570; 0402 animal and dairy science; 04 agricultural and veterinary sciences; SV Veterinary science / állatorvostudomány

AbstractThe authors aimed to investigate eight strains of Escherichia coli (E. coli) strains from Hungarian layer flocks for antimicrobial resistance genes (ARG), using metagenomic methods. The strains were isolated from cloacal swabs of healthy adult layers. This study employed shotgun sequencing-based genetic and bioinformatic analysis along with determining phenotypic minimum inhibitory concentrations. A total of 59 ARGs were identified in the eight E. coli isolates, carrying ARGs against 15 groups of antibiotics. Among these, 28 ARGs were identified as transferable. Specifically, four ARGs were plasmid-derived, 18 ARGs were phage-derived and an additional six ARGs were predicted to be mobile, contributing to their mobility and potential spread between bacteria.