Use of sodium dodecyl sulfate to improve tuberculosis sputum smear microscopy

• DOI: 10.15641/ghi.v2i2.824
• Publication Date: 2019-12-03T10:42:17Z
• Authors: Yéya dit Sadio Sarro; Ousmane Kodio; ; Alisha Kumar; ; Bassirou Diarra; ; Bocar Baya; ; Seydou Diabate; ; Bourahima Kone; ; Fanta Sanongo; ; Mohamed Tolofoudie; ; Amadou Somboro; ; Gagni Coulibaly; ; Boureima Degoga; ; Mahamadou Kone; ; Bindongo P P Dembele; ; Issiaka Camara; ; Moumine Sanogo; ; Antime C G Togo; ; Nadie Coulibaly; ; Fatimata Diallo; ; Etienne Dembele; ; Brehima Diakite; ; Doumbia Seydou; ; Oluwatoyin P Popoola; ; Souleymane Diallo; ; Jane Louise Holl; ; Chad J Achenbach; ; Robert Leo Murphy; ; Sally McFall; ; Mamoudou Maiga
• Keywords: Sodium dodecyl sulfate; Biosafety

Sputum smear microscopy (SSM), the most widely available tool for tuberculosis (TB) detection, has limited performance in paucibacillary patients and requires highly experienced technicians. The objective of this study was to determine whether addition sodium dodecyl sulfate (SDS), a detergent that thins sputum, at 4% 10%, improves detection acid-fast bacilli (AFB), clarity slides, biosafety technique. Thirty participants with presumptive TB were enrolled. Three independent, blinded technicians examined slides. Regular sputum concentrated AFB culture used as standard control methods. also performed before after 10% SDS safety analysis. We found neither SSM nor improved test’s performance. However, slides SDS, compared prepared without had significantly better scores. SDS-prepared samples all negative. While adding does not improve it biosafety. Where are scarce, especially low resource settings, use may enhance ease slide reading microscopy.