Bovine papillomavirus productive infection in cell cultures: First evidences

• DOI: 10.15761/vrr.1000110
• Publication Date: 2017-06-27T06:00:07Z
• Authors: Rodrigo Pinheiro ...; Thatiana Correa d...; Silvio Roberto Co...; Jacqueline Mazzuc...; Diego Grando Módolo; Suelly Muro Reis ...; Thaylene Alexandr...; Roberta Fiusa Mag...; Diva Denelle Spad...; Rodrigo Franco de...; Willy Beçak; Rita de Cassia St...
• Keywords: Bovine papillomavirus

Bovine papillomavirus (BPV) is the etiological agent of bovine papillomatosis (BP), infectious disease, characterized by presence multiples papillomas that can regress spontaneously or progress to malignances.Although recognized as mutagen, BPV action following cancer initiation remains few explored, since studies about progression and metastasis are based on cell cultures.The lack attention in vitro models a reflection replication paradigm, which dependent epithelium differentiation.Since 2008, we have explored potential lines derived from BPV-infected neoplasms model study oncogenic process.In this study, described productive infection cutaneous papilloma, fibropapilloma esophageal carcinoma (EC) DNA sequences were previously detected PCR.Considering immunodetection L1 capsid protein main evidence infection, analyzed expression immunofluorescence flow cytometry.Results showed EC, but not cells BPV-free normal skin.We also observed spherical electron-dense particles, with 41.02-61.94nm diameter cytoplasmic vesicles sixth passage being compatible expected morphology.Cells skin, turn, membranous particles up 75.00 nm morphology.These results suggest neoplasm, reinforcing these useful viral biology pathogenesis.