On-line concentration of biogenic amines by capillary electrophoresis using synthesized covalent coating based on imidazolium ionic liquid

• DOI: 10.15826/analitika.2017.21.1.006
• Publication Date: 2017-04-05T13:55:15Z
• Authors: Е. А. Kolobova; L. A. Kartsova; E. A. Bessonova; A. V. Kravchenko
• Keywords: КОВАЛЕНТНЫЕ ПОКРЫТИЯ; БИОГЕННЫЕ АМИНЫ; COVALENT COATING; ON-LINE КОНЦЕНТРИРОВАНИЕ; IONIС LIQUIDS; BIOGENIC AMINES; ON-LINE CONCENTRATION; ИОННЫЕ ЖИДКОСТИ; 01 natural sciences; 0104 chemical sciences

Covalent coatings of internal quartz capillary walls based on the ionic liquids were synthesized within this research. The synthesis covalent coating included a step silylation followed by functionalization with imidazole and 1-bromobutane. resulting created anodic electroosmotic flow (EOF) at pH = 2. range which do not change their properties was 2.0-5.5. possibilities different ways on-line concentration ( large volume sample stacking, head-column field-amplified sweeping, sweeping in combination electrostacking) catecholamines examined. Stacking efficiency factors (SEF) 105-135 detection limits decreased to 11-17 ng/ml for analytes under stacking. Under conditions, sodium dodecyl sulfate (micelle forming agent) sorbed modified walls, provided negative charging surface acted as strong cation exchanger. This fact led additional reserves separation selectivity catecholamines. electrostacking, values SEF above 1000 reduced 1-2 ng/ml. An approbation established patterns analysis urine samples carried out. Keywords: coating, ioniс liquids, biogenic amines, (Russian) DOI: http://dx.doi.org/10.15826/analitika.2017.21.1.006 E.A. Kolobova 1,2 , L.A. Kartsova 1 Bessonova A.V. Kravchenko Saint-Petersburg State University, Institute Chemistry, 26 Universitetskii prospect, St. Petersburg, Petergof 198504, Russian Federation 2 Nikiforov Center Emergency Radiation Medicine, 54 Optikov st., 199034,