First isolation of West Nile virus in Brazil

• DOI: 10.1590/0074-02760180332
• Publication Date: 2019-02-05T09:25:50Z
• Authors: Lívia Caricio Mar...; Eliana Vieira Pin...; Livia Medeiros Ne...; Sandro Patroca da...; Ana Cecília Ribei...; Jamilla Augusta d...; Daniele Barbosa d...; Arnaldo Jorge Mar...; Ermelinda do Rosá...; Marialva Tereza F...; Jedson Ferreira C...; Marcos Antônio Co...; Gilton Luiz Almada; Alessandro Pecego...; Maria Guadalupe D...; Gilsa Aparecida P...; Jannifer Oliveira...; Juarez Antonio Si...; Valéria Lima Carv...; Pedro Fernando da...
• Keywords: Encephalomyelitis, Equine; Male; Reverse Transcriptase Polymerase Chain Reaction; Infectious and parasitic diseases; RC109-216; Microbiology; Immunohistochemistry; QR1-502; 3. Good health; Phylogeography; 03 medical and health sciences; 0302 clinical medicine; Animals; RNA, Viral; Original Article; Horse Diseases; Horses; West Nile virus; horses; Brazil; West Nile Fever

Serological evidence of West Nile virus (WNV) infection has been reported in different regions Brazil from equine and human hosts but the had never isolated country.We sought to identify viral etiology encephalitis Espírito Santo state.We performed culture C6/36 cells, molecular detection WNV genome, histopathology immunohistochemistry horse cerebral tissue. We also carried out sequencing, phylogenetic analysis clock.Histopathologic tissue showed injury related was confirmed by immunohistochemistry. The detected reverse transcription quantitative polymerase chain reaction (RT-qPCR) brain subsequently cells. full-length genome sequenced showing strain belongs lineage 1a. clock indicated that Brazilian share same common ancestor were circulating US during 2002-2005.Here we report first isolation a with neurologic disease, which clustered into 1a others strains beginning 2000's decade.