Anti-Inflammatory Agent Indomethacin Reduces Invasion and Alters Metabolism in a Human Breast Cancer Cell Line

• DOI: 10.1593/neo.06673
• Publication Date: 2007-03-24T16:11:19Z
• Authors: Ellen Ackerstaff; Barjor Gimi; Dmitri Artemov; Zaver M. Bhujwalla
• Keywords: 0301 basic medicine; Growth Differentiation Factor 15; Magnetic Resonance Spectroscopy; Indomethacin; phospholipid metabolism; Breast Neoplasms; 03 medical and health sciences; Breast cancer; indomethacin; Cell Line, Tumor; Humans; Neoplasm Invasiveness; RC254-282; Triglycerides; Anti-Inflammatory Agents, Non-Steroidal; Neoplasms. Tumors. Oncology. Including cancer and carcinogens; invasion; 3. Good health; Gene Expression Regulation, Neoplastic; Prostaglandin-Endoperoxide Synthases; Phosphatidylcholines; Cytokines; Matrix Metalloproteinase 2; Female; magnetic resonance (MR)

Hostile physiological environments such as hypoxia and acidic extracellular pH, which exist in solid tumors, may promote invasion metastasis through inflammatory responses formation of eicosanoids. Here, we have investigated the effects antiinflammatory agent indomethacin on metabolism human breast cancer cell line MDAMB-435 Dulbecco's Modified Eagles (DME)-based or Roswell Park Memorial Institute (RPMI)-based medium, using a magnetic resonance-compatible assay. Indomethacin treatment significantly reduced MDA-MB-435 cells independent culture perfusion conditions examined. Significant changes were detected levels intracellular choline phospholipid metabolites triglyceride (TG) concentrations these cells, depending basal medium used. Additionally, genetic profiling grown treated with low-dose an RPMI-based revealed upregulation several genes implicating cyclooxygenaseindependent targets indomethacin. These data confirm ability anti-inflammatory to reduce demonstrate, conditions, that indomethacin-induced decrease is associated metabolism, TG gene expression.