C-Kit and Its Ligand Stem Cell Factor: Potential Contribution to Prostate Cancer Bone Metastasis

• DOI: 10.1593/neo.08618
• Publication Date: 2015-04-23T13:11:52Z
• Authors: Christoph Wiesner; Sanaa M. Nabha; R. Daniel Bonfil; Emanuel Burck Dos...; Hamilto Yamamoto; Hong Meng; Sebastian W. Melc...; Fernando Bittinger; Joachim W. Thüroff; Robert L. Vessella; Michael L. Cher
• Keywords: Proto-Oncogene Proteins c-kit

The tyrosine kinase receptor c-kit and its ligand stem cell factor (SCF) have not been explored in prostate cancer (PC) bone metastasis. Herein, we found that three human PC lines marrow stromal cells express a membrane-bound SCF isoform release soluble SCF. Bone revealed strong expression of c-kit, whereas showed very low levels the or did it all. Using an experimental model metastasis, intraosseous tumors formed by otherwise c-kit–negative PC3 strongly expressed as demonstrated using immunohistochemical Western blot analyses. Subcuta-neous were, however, c-kit–negative. Both subcutaneous were positive for Immunohistochemical analysis specimens frequency epithelial was 5% benign prostatic hyperplasia, 14% primary PC, 40% metastases, suggesting overall trend increased clinical progression. Stem more than 80% all cases. Our data suggest microenvironment up-regulates on cells, favoring their expansion.